In addition, seven components of the decarboxylation module were analyzed including NAD-dependent malic enzyme ( McNAD-ME1, McNAD-ME2), NADP-dependent malic enzyme ( McNADP-ME1, NADP-ME2), pyruvate, orthophosphate dikinase ( McPPDK), pyruvate, orthophosphate dikinase-regulatory protein ( McPPDK-RP), and phosphoenolpyruvate carboxykinase ( McPEPCK). Six components of the carboxylation module were analyzed including beta-carbonic anhydrase ( McBCA2), phosphoenolpyruvate carboxylase ( McPEPC1), phosphoenolpyruvate carboxylase kinase ( McPPCK1), NAD-dependent malate dehydrogenase ( McNAD-MDH1, McNAD-MDH2), and NADP-dependent malate dehydrogenase ( McNADP-MDH1). Here, we isolated and analyzed the subcellular localizations of 13 enzymes and regulatory proteins of the C 4 metabolism cycle of CAM from the common ice plant in stably transformed Arabidopsis thaliana. To optimize the success of CAM Biodesign approaches, the functional analysis of individual C 4 metabolism cycle genes is necessary to identify the essential genes for robust CAM pathway introduction. Introducing the CAM photosynthetic machinery into C 3 (or C 4) photosynthesis plants (CAM Biodesign) represents a potentially breakthrough strategy for improving WUE while maintaining high productivity. Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV, United StatesĬrassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that exploits a temporal CO 2 pump with nocturnal CO 2 uptake and concentration to reduce photorespiration, improve water-use efficiency (WUE), and optimize the adaptability of plants to hotter and drier climates.Sung Don Lim, Sojeong Lee, Won-Gyu Choi, Won Cheol Yim and John C.
0 kommentar(er)
